Journal: JVS-Vascular Science
Article Title: Zinc deficiency impairs ischemia-induced angiogenesis
doi: 10.1016/j.jvssci.2021.09.023
Figure Lengend Snippet: Zinc-deficient ( ZD ) mice showing reductions in the rates of perfusion recovery and capillary vessel formation in ischemic limbs. A, Representative laser Doppler blood flow (LDBF) images of the ischemic limb of ZD mice or control wild-type (WT) mice. A low perfusion signal ( dark blue ) was observed in the ischemic hindlimb of ZD mice. In contrast, a high perfusion signal ( red ) was detected in the control WT mice at postoperative days 14, 21, and 28. B, Quantitative analysis of the ischemic/nonischemic LDBF ratio in the ZD mice or control WT mice before surgery and at different points after surgery. Results are presented as the mean ± standard deviation (n = 8 in each group). ∗ P < .05 vs ZD mice. C, Fluorescence staining of ischemic tissues with anti-CD31 monoclonal antibody ( red ) on postoperative day 28. D and E, Quantitative analysis of capillary density in ZD mice or control WT mice on postoperative day 28. Capillary density expressed as the number of capillaries per high power field (×400) ( Left ) and capillaries per muscle fiber ( Right ). F, Messenger RNA (mRNA) levels of vascular endothelial growth factor (VEGF) in the ischemic muscle in the ZD mice or control WT mice on postoperative day 28. mRNA levels were measured using the real-time polymerase chain reaction (PCR) method (n = 8 in each group). All results were normalized to glyceraldehyde 3-phosphate dehydrogenase. ∗ P < .01 vs control. Results presented as mean ± standard deviation (n = 8 in each group).
Article Snippet: The hindlimb blood flow was measured using a laser Doppler blood flow (LDBF) analyzer (Moor LDI; Moor Instruments Inc, Wilmington, Del) immediately before surgery and on postoperative days 3, 7, 14, and 28.
Techniques: Control, Standard Deviation, Fluorescence, Staining, Real-time Polymerase Chain Reaction